Alicyclobacillus pomorum sp. nov., a novel thermo-acidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus

Publisher Website
Google Scholar
Abstract
A thermo-acidophilic endospore-forming bacterium was isolated from a mixed fruit juice. The organism, strain 3AT, was rod-shaped, grew aerobically at 30–60 °C (optimum 45–50 °C), pH 3·0–6·0 (optimum pH 4·0–4·5) and produced acid from various sugars. It contained menaquinone-7 as the major isoprenoid quinone. The G+C content of the DNA was 53·1 mol%. The predominant cellular fatty acids of the strain were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0, but ω-alicyclic fatty acids, which are characteristic of the genus Alicyclobacillus, were not found in the strain. Phylogenetic analyses based on both 16S rRNA and gyrB (DNA gyrase B subunit gene) gene sequences showed that strain 3AT falls into the Alicyclobacillus cluster, validated by significant bootstrap values. However, strain 3AT did not show a close relationship to the other species of the cluster. The level of 16S rDNA similarity between strain 3AT and other strains of the cluster was between 92·5 and 95·5 %. The level of gyrB sequence similarity between strain 3AT and other strains of the cluster was between 68·5 and 74·4 %. DNA–DNA hybridization values between strain 3AT and phylogenetically related strains of the genera Alicyclobacillus, Bacillus and Sulfobacillus were under 13 %, indicating that strain 3AT represents a distinct species. On the basis of these results, strain 3AT should be classified as a novel Alicyclobacillus species. The name Alicyclobacillus pomorum is proposed for this organism. The type strain of Alicyclobacillus pomorum is strain 3AT (=DSM 14955T=IAM 14988T).

This publication has 39 references indexed in Scilit: