A general priming system employing only dnaB protein and primase for DNA replication.
Open Access
- 1 September 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (9) , 4308-4312
- https://doi.org/10.1073/pnas.76.9.4308
Abstract
Priming of phage .vphi.X174 DNA synthesis is effected simply by dnaB protein and primase when the DNA is not coated by single-strand binding protein (SSB). The 5 prepriming proteins (n, n'', n", i and dnaC protein) required for priming a SSB-coated .vphi.X174 DNA circle are dispensable. The dnaB protein-priming system is also active on uncoated phage G4 and M13 DNA and on poly(dT). Multiple RNA primers, 10-60 nucleotides long, are transcribed with patterns distinctive for each DNA template. Formation of a stable dnaB protein.cntdot.DNA complex in the presence of primase and ATP supports the hypothesis that dnaB protein provides a mobile replication promoter signal for primase.Keywords
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