PROTEIN SYNTHESIS IN DARK-GROWN BEAN LEAVES

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Abstract
RuDP carboxylase was present in 4-day-old, dark-grown bean leaves. The enzyme increased during subsequent dark growth and in 11 days reached one-third of the specific activity of a light-grown control. Preparative disc electrophoresis of the soluble protein revealed a zone symmetrical with RuDP carboxylase. Increases in RuDP carboxylase during dark growth were matched by corresponding increases in this protein zone. The correlation indicated that the enzyme accounted for 45% of the soluble protein in 11-day-old, light-grown leaves. The formation of peroxidase and catalase was much slower than the formation of RuDP carboxylase during dark growth.