Specific detection of non‐functional human P2X7 receptors in HEK293 cells and B‐lymphocytes
- 22 February 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 538 (1-3) , 159-162
- https://doi.org/10.1016/s0014-5793(03)00172-8
Abstract
P2X7 receptor/channels mediate ATP-induced apoptosis in a range of cells including lymphocytes. HEK293 cells were transfected with wild-type human P2X7 receptor or site-directed mutant constructs (K193A, K311A and E496A) known to be non-functional from measurements of barium/ethidium influx in the presence of ATP or 2′,3′-O-(4-benzoylbenzoyl)-ATP. An antibody was designed against an epitope from a loop adjacent to the extracellular ATP site. The epitope was unavailable in cells expressing normal functional surface receptors. Non-functional surface receptors as well as intracellular receptors selectively bound the antibody. So did B-lymphocytes from chronic lymphocytic leukemia patients expressing non-functional (E496A) mutant receptorKeywords
This publication has 21 references indexed in Scilit:
- Species- and agonist-dependent differences in the deactivation-kinetics of P2X 7 receptorsNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 2001
- A Glu-496 to Ala Polymorphism Leads to Loss of Function of the Human P2X7 ReceptorJournal of Biological Chemistry, 2001
- The Distribution of P2X Receptor Clusters on Individual Neurons in Sympathetic Ganglia and Their Redistribution on Agonist ActivationPublished by Elsevier ,2000
- Pharmacological characterization of ATP‐ and LPS‐induced IL‐1β release in human monocytesBritish Journal of Pharmacology, 1999
- P2X1 and P2X3 receptors form stable trimers: a novel structural motif of ligand-gated ion channelsThe EMBO Journal, 1998
- Binding of monoclonal antibody 4B1 to homologs of the lactose permease ofEscherichia coliProtein Science, 1997
- The Permeabilizing ATP Receptor, P2X7Journal of Biological Chemistry, 1997
- The Cytolytic P 2Z Receptor for Extracellular ATP Identified as a P 2X Receptor (P2X 7 )Science, 1996
- The ATP4− receptor-operated ion channel of human lymphocytes: Inhibition of ion fluxes by amiloride analogs and by extracellular sodium ionsArchives of Biochemistry and Biophysics, 1992
- NMR evidence for an early framework intermediate on the folding pathway of ribonuclease ANature, 1988