NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A
- 1 October 1988
- journal article
- Published by Springer Nature in Nature
- Vol. 335 (6192) , 694-699
- https://doi.org/10.1038/335694a0
Abstract
The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional 1H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure.Keywords
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