Segment α 182‐198 of Torpedo californica acetylcholine receptor contains a second toxin‐binding region and binds anti‐receptor antibodies
- 7 April 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 199 (1) , 68-74
- https://doi.org/10.1016/0014-5793(86)81225-x
Abstract
The area around Cys-192 and Cys-193 is thought to be a functionally important part of the α-subunit of the acetylcholine receptor. We have synthesized peptide α 182-198 of the α-chain of the Torpedo californica acetylcholine receptor and investigated the binding to the peptide of α-bungarotoxin, cobratoxin and antibodies raised against acetylcholine receptor. The results showed that the synthetic peptide α 182-198 contains a second toxin-binding region and also binds a considerable fraction of anti-receptor antibodies. We also report here the toxin-binding activity of synthetic peptide a 125–148 of the human acetylcholine receptor which has been previously localized as a toxin-binding region in the α-chain of the Torpedo receptor.Keywords
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