Location of functional regions of acetylcholine receptor α-subunit by site-directed mutagenesis

31 January 1985

journal article
research article
Published by Springer Nature in Nature

Vol. 313 (6001) , 364-369
https://doi.org/10.1038/313364a0

Abstract

The availability of cloned cDNAs encoding the four subunits of the Torpedo acetylcholine receptor, which can be expressed to make functional receptors in Xenopus oocytes, has made possible a detailed investigation of the functions of the different structural components of the receptor. The functional analysis of receptors with α-subunits altered at specific sites by site-directed mutagenesis of the cDNA has allowed the location of specific regions of the α-subunit molecule involved in acetylcholine binding and forming a transmembrane ionic channel.

Keywords

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