PHYSICOCHEMICAL PROPERTIES OF PHAGES FOR AGROBACTERIUM RADIOBACTER

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Abstract
Plaques of phages PR-1001, PS-192, PR-590a, and PsR-1012 were clear, without halos, and with definite edges. Phage heads were approximately 60 mμ in diameter with short blunt tails. Phage titers in nutrient broth were stable during 452 days storage at 4 °C but were decreased considerably at 25 °C. After 46 days at 37 °C no surviving particles of any of the phages were detected. Ultraviolet inactivation of particles was not a first-order reaction. The pH stability range was between pH 7 and 11. Inactivation at 50 °C and 55 °C was least in saline, more marked in nutrient broth, and most severe in distilled water. Protection against inactivation was a function of the mono- and di-valent cation concentration. Separately the cations provided insignificant protection. NH4+ in combination with Ca++ or Mg++ inactivated phage particles as did also Cu++, Hg++, Al+++, and Fe+++ in solutions of NaCl. Heat-inactivated particles showed collapsed heads that indicated a loss in DNA.

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