Effects of Estradiol on Prostaglandin F2αLevels in Primary Monolayer Cultures of Epithelial Cells from Human Proliferative Endometrium*

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Abstract
Primary monolayer cultures of glandular epithelial or stromal cells isolated from human proliferative endometrium release prostaglandin F2.alpha. (PGF2.alpha.) in the medium. In epithelial cell cultures maintained in medium containing charcoal-stripped calf serum, estradiol (10-8 M) increased the levels of PGF2.alpha. 2.5- to 5-fold during the first 24 h and as much as 40-fold during the 2nd and 3rd days in culture. A similar response was seen with estradiol at a concentration of 10-10 M. The response to estradiol was only about half as great in medium containing untreated calf serum. Preliminary measurements of PGF2.alpha. levels in stromal cell cultures showed that estradiol did not increase PGF2.alpha. levels in the medium and even reduced it in some preparations. The estradiol-stimulated increase in PGF2.alpha. levels in the epithelial cell cultures contrasts with the lack of responsiveness of proliferative endometrium in organ culture. The ability of glands to respond to estradiol apparently is repressed in proliferative endometrium.