METHOD FOR ISOLATION OF GARDNERELLA VAGINALIS (HAEMOPHILUS VAGINALIS) Characterization of Isolates by Gas Chromatography
- 1 September 1982
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Series B: Microbiology
- Vol. 90B (1-6) , 89-93
- https://doi.org/10.1111/j.1699-0463.1982.tb00087.x
Abstract
A method for the isolation of G. vaginalis (H. vaginalis) [a suspected causative agent of vaginitis] is presented. Bacteria isolated from 48-h cultures grown on human blood agar were identified by means of .beta.-hemolysis, colony morphology, sensitivity to antimicrobial agents, oxidase and catalase reactions. Thirty-eight clinical isolates and 1 test strain were examined for fatty acid composition. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids. Also present, but in minor quantities, were myristic (14:0), hexadecenoic (16:1) and octadecadienoic (18:2) acids. Only insignificant differences between isolates could be detected. No hydroxy fatty acids commonly found in gram-negative bacteria were encountered. Gas chromatographic analysis of G. vaginalis revealed a characteristic and relatively simple pattern. The results support the use of the isolation method, which provides conditions highly selective for G. vaginalis.Keywords
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