Production, Purification and Characterization of HYI, an Anti-yeast Substance, Produced byHansenula saturnus

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Abstract
An anti-yeast substance, HYI, was produced by Hansenula saturnus. Production of HYI under aerobic incubation conditions took place in parallel with yeast cellular growth. HYI was concentrated by ultrafiltration from the culture filtrate and purified by ion-exchange chromatography followed by gel filtration. It was then crystallized. HYI migrated as a single protein band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis and had a molecular weight of about 8500. Anthrone positive compounds could not be detected in HYI. The isoelectric point of HYI was pH 5.80 ± 0.05. The activity of HYI was unaffected by heating at 60°C for 1 hr. It showed a broad anti-yeast spectrum.