Lipoxygenase in Sweet Corn Germ: Isolation and Physicochemical Properties
- 1 September 1995
- journal article
- Published by Wiley in Journal of Food Science
- Vol. 60 (5) , 1029-1033
- https://doi.org/10.1111/j.1365-2621.1995.tb06286.x
Abstract
Off‐flavor and off‐aroma development, which may be catalyzed by lipoxygenase (LPO), are common in frozen stored sweet corn. Lipoxygenase activity in the germ fraction of sweet com (Zea nruys L. cv. Jubilee) was determined and compared with that in the degermed fraction. Lipoxygenase activity/g germ was about three times greater than that of the degermed fraction, Optimized procedures for isolation of lipoxygenase from the germ fraction were developed. Lipoxygenase was isolated by preparation as an acetone powder, extraction with 0.2M TrisHCI, pH 8.0 (4°C), fractionation with 40–60% saturated ammonium sulfate and dialysis. Optimum pH was 6–7 and temperature 50°C for activity of partially purified lipoxygenase. The enzyme appeared stable at pH 5–8 and ∼90% of original activity was inactivated after heating in pH 7 buffer at 70°C for 3 min.Keywords
This publication has 44 references indexed in Scilit:
- Purification of lipoxygenase and hydroperoxide dehydrase in flaxseeds: Interaction between these enzymatic activitiesBiochemical and Biophysical Research Communications, 1992
- Purification and characterization of two lipoxygenase isoenzymes from germinating barleyBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Lipoxygenases from Zea mays L. Purification and physicochemical characteristicsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- Enzymes Responsible for Off-Flavor and Off-Aroma in Blanched and Frozen-Stored Vegetables1Biotechnology and Applied Biochemistry, 1989
- The behaviour of green bean lipoxygenase on chromatography and isoelectric focusingFood Chemistry, 1989
- Iron environment in soybean lipoxygenase-1Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
- Metabolism of Linoleic Acid by Barley Lipoxygenase and Hydroperoxide IsomerasePlant Physiology, 1981
- Partial purification and study of some properties of rice germ lipoxygenase.Agricultural and Biological Chemistry, 1980
- Stereospecificity of the hydrogen abstraction at carbon atom n-8 in the oxygenation of linoleic acid by lipoxygenases from corn germs and soya beansBiochemical and Biophysical Research Communications, 1972
- Positional specificity of corn germ lipoxygenase as a function of pHBiochemical and Biophysical Research Communications, 1972