Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum β-Lactamases
- 1 August 2006
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (8) , 3008-3011
- https://doi.org/10.1128/jcm.02576-05
Abstract
A pyrosequencing technique was used for identification of extended-spectrum β-lactamases (ESBLs) of GES type. These β-lactamases are isolated increasingly emerging in gram-negative bacteria worldwide. This rapid and reliable identification method is interesting, since GES variants, including not only expanded-spectrum cephalosporins but also carbapenems, cephamycins, and monobactams, are the only ESBLs that possess different hydrolysis profiles.Keywords
This publication has 24 references indexed in Scilit:
- Integron-Encoded GES-Type Extended-Spectrum β-Lactamase with Increased Activity toward Aztreonam in Pseudomonas aeruginosaAntimicrobial Agents and Chemotherapy, 2005
- Spread of novel expanded-spectrum β-lactamases in Enterobacteriaceae in a university hospital in the Paris area, FranceClinical Microbiology & Infection, 2005
- Pyrosequencing-based approach for rapid detection of rifampin-resistant Mycobacterium tuberculosisDiagnostic Microbiology and Infectious Disease, 2005
- Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR AssayAntimicrobial Agents and Chemotherapy, 2004
- Sequence-Selective Recognition of Extended-Spectrum β-Lactamase GES-2 by a Competitive, Peptide Nucleic Acid-Based Multiplex PCR AssayAntimicrobial Agents and Chemotherapy, 2004
- Molecular Characterization of a Cephamycin-Hydrolyzing and Inhibitor-Resistant Class A β-Lactamase, GES-4, Possessing a Single G170S Substitution in the Ω-LoopAntimicrobial Agents and Chemotherapy, 2004
- Emergence of the Extended-Spectrum β-Lactamase GES-1 in a Pseudomonas aeruginosa Strain from Brazil: Report from the SENTRY Antimicrobial Surveillance ProgramAntimicrobial Agents and Chemotherapy, 2004
- Novel GES/IBC extended-spectrum β-lactamase variants with carbapenemase activity in clinical enterobacteriaFEMS Microbiology Letters, 2004
- Novel GES/IBC extended-spectrum ?-lactamase variants with carbapenemase activity in clinical enterobacteriaFEMS Microbiology Letters, 2004
- Outbreak of GES-1 β-Lactamase-Producing Multidrug-Resistant Klebsiella pneumoniae in a University Hospital in Lisbon, PortugalAntimicrobial Agents and Chemotherapy, 2003