In situ hybridization to somatic metaphase chromosomes of potato
- 1 September 1988
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 76 (3) , 420-424
- https://doi.org/10.1007/bf00265343
Abstract
An in situ hybridization procedure was developed for mitotic potato chromosomes by using a potato 24S rDNA probe. This repetitive sequence hybridized to the nucleolar organizer region (NOR) of chromosome 2 in 95%–100% of the metaphase plates. Another repetitive sequence (P5), isolated from the interdihaploid potato HH578, gave a “ladderpattern” in genomic Southern's of Solanum tuberosum and Solanum phureja, but not in those of Solanum brevidens and two Nicotiana species. This sequence hybridized predominantly on telomeric and centromeric regions of all chromosomes, although chromosomes 7, 8, 10 and 11 were not always labeled clearly.This publication has 21 references indexed in Scilit:
- Karyotype variation in aminoethylcysteine resistant cell and callus cultures and regenerated plants of a dihaploid potato (Solanum tuberosum)Plant Cell Reports, 1987
- Cloning in Escherichia coli of the gene specifying the DNA-entry nuclease of Bacillus subtilisGene, 1987
- An Enzymic Method of Preparing Plant Chromosomes forIn SituHybridizationStain Technology, 1986
- Somaclonal variation in Solanum tuberosum detected at the molecular levelTheoretical and Applied Genetics, 1985
- 2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequencesExperimental Cell Research, 1984
- An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gelsAnalytical Biochemistry, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- A rapid alkaline extraction procedure for screening recombinant plasmid DNANucleic Acids Research, 1979
- SOME NEW ASPECTS OF EFFICIENCY OF ELECTRON MICROSCOPIC AUTORADIOGRAPHY WITH TRITIUMJournal of Histochemistry & Cytochemistry, 1970
- FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONSProceedings of the National Academy of Sciences, 1969