Somaclonal variation in Solanum tuberosum detected at the molecular level
- 1 December 1985
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 71 (3) , 500-505
- https://doi.org/10.1007/bf00251196
Abstract
The many reports of phenotypic variation among plants regenerated from tissue culture suggest underlying alterations at the DNA level. This hypothesis was tested with protoplast-derived Solanum tuberosum plants. Random potato-DNA clones were used to probe the genome of individual plants at specific sites. Two out of twelve plants were shown to be variant by Southern-hybridisation with one of the tester-clones. As this clone turned out to represent 25S-rDNA, both somaclonal variants can be regarded as mutants deficient in ribosomal RNA-genes.This publication has 29 references indexed in Scilit:
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