Measurement of K vitamins in human and animal feces by high-performance liquid chromatography with fluorometric detection.
- 1 January 1986
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (10) , 4322-4326
- https://doi.org/10.1248/cpb.34.4322
Abstract
A high-performance liquid chromatographic (HPLC) method was developed for measuring endogenous K vitamins in human and animal feces, and these vitamins in human feces were also identified by mass spectrometry (MS). K vitamins extracted from human and animal feces were purified by thin-layer chromatography (TLC) and then measured by reversed-phase HPLC with fluorometric detection. Vitamin K1 (VK1) and menaquinone (MK)-4 to MK-15 were found and their amounts were determined in human, monkey and rabbit feces. K vitamins extracted from human feces were purified successively by column chromatography, TLC, reversed-phase HPLC and normal-phase HPLC, and then identified as VK1 and MK-4 to MK-15 by MS, confirming the validity of the HPLC method.This publication has 2 references indexed in Scilit:
- Measurement of K vitamins in human and animal plasma by high-performance liquid chromatography with fluorometric detection.CHEMICAL & PHARMACEUTICAL BULLETIN, 1986
- Fluorometric Determination of Phylloquinone and Menaquinone-4 in Biological Materials using High Performance Liquid ChromatographyYAKUGAKU ZASSHI, 1979