Quantitation of Collagen Types I and III during Wound Healing in Rat Skin

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Abstract
The collagen types synthesized during early wound healing have not been quantitatively analyzed. Open wounds were made on the backs of Sprague-Dawley rats and analyzed at various times for newly synthesized collagen Types I and III. Biopsies were incubated in tissue culture medium with [3H]proline and collagen was extracted and purified prior to separation on an A5-M column. Type III collagen was increased significantly at 10 h compared to all other times through day 12. By 24 h, the percentage Type III collagen returned to a normal skin value of 20%. The early appearance of Type III collagen is associated with an early increase in collagen synthesis and may function in providing initial wound structure and support for subsequent healing events.