Rapid and simple determination of selenium in blood serum by inductively coupled plasma–mass spectrometry (ICP–MS)

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Abstract
An inductively coupled plasma mass spectrometer (ICP–MS) with a rapid sample-preparative procedure was used for the determination of selenium in blood serum. Blood serum was prepared by dilution in an acidic solution consisting of nitric acid (1%), X-triton (0.1%) and 1-butanol (0.8%). A calibration curve was established for 1–40 µg mL−1 (r2>0.99). The limit of detection was 0.5 µg mL−1. Repeatability and intermediate precision were satisfactory with relative standard deviations (RSD) of 2.0% and 3.2%, respectively. This method was easily applied to reference materials with satisfactory accuracy. Good correlation (r2=0.96) was observed between ICP–MS and atomic absorption spectrometry (AAS) for the determination of 82Se in blood serum from 23 patients. These results suggest that the sample preparative procedure coupled with ICP–MS can be used for the routine determination of 82Se in human blood serum.

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