KCO1 is a component of the slow‐vacuolar (SV) ion channel
- 17 December 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 511 (1-3) , 28-32
- https://doi.org/10.1016/s0014-5793(01)03273-2
Abstract
The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels.Keywords
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