STUDIES ON LIPASE I. ON THE ACTIVATION OF PANCREAS LIPASE

Publisher Website
Google Scholar
Abstract
Lipase from pig pancreas was used with olive oil or triacetin as substrates and polyvinyl alcohol or Na carboxymethylcellulose as emulsifiers. Addition of dicarboxylic amino acids or diamino acids increased the enzymic activity by about 50%. The accelerating effect of histidine exceeded 100%. Similar activation of lipase was observed with succinic acid, citric acid, and maleic acid, whereas fumaric acid was inactive. Lipase soln. incubated at pH 9.2 lost 50% of its activity. Restoration to 85ft of the original activity or prevention of the loss of activity to a similar degree resulted from addition of aspartic acid to the enzyme soln.