Glycogen content of mouse hemidiaphragm as a biologic indicator of insulin action

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Abstract
The mouse hemidiaphragm insulin assay utilizes glycogen content as the biologic indicator of insulin activity. It is statistically valid and has a mean index of precision of 0.22. The effects of alterations in the incubation medium showed that hyperosmolarity, hypo-osmolarity, and inadequate oxygenation reduced the glycogen content. There was a linear increase in glycogen content when it was plotted against the log of glucose concentration from 3 to 20 mg/ml. At these glucose concentrations, maximal insulin stimulation increased glycogen content 50–100%. Optimal pH was 7.34–7.53 and optimal incubation time was 90 min. Insulin activity of native serum (NSIA) was low in peripheral vein and much higher in pancreatic vein. Insulin activity in acid alcoholic treated dialyzed serum (TSIA) was much higher than NSIA in peripheral vein and significantly higher in pancreatic vein. NSIA was neutralizable; the additional activity made manifest by treatment was not. The effect of NSIA, TSIA, and standard insulin decreased in parallel manner with dilution.

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