Ischemia Opens Neuronal Gap Junction Hemichannels
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- 12 May 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 312 (5775) , 924-927
- https://doi.org/10.1126/science.1126241
Abstract
Neuronal excitotoxicity during stroke is caused by activation of unidentified large-conductance channels, leading to swelling and calcium dysregulation. We show that ischemic-like conditions [O2/glucose deprivation (OGD)] open hemichannels, or half gap junctions, in neurons. Hemichannel opening was indicated by a large linear current and flux across the membrane of small fluorescent molecules. Single-channel openings of hemichannels (530 picosiemens) were observed in OGD. Both the current and dye flux were blocked by inhibitors of hemichannels. Therefore, hemichannel opening contributes to the profound ionic dysregulation during stroke and may be a ubiquitous component of ischemic neuronal death.Keywords
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