Materials for enhancing cell adhesion by immobilization of cell‐adhesive peptide
- 1 November 1991
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 25 (11) , 1325-1337
- https://doi.org/10.1002/jbm.820251102
Abstract
Materials to enhance cell adhesion were synthesized by surface integration of peptide, Arg-Gly-Asp-Ser(RGDS), which is an active-site sequence of cell-adhesive proteins. Polystyrene film was glowdischarged and graft-copolymerized with acrylic acid. Then the peptide was immobilized to the poly(acrylic acid) grafts by using water-soluble carbodimide. The cell-adhesive activity of the RGDS-immobilized film increased with increasing amount of immobilized peptide, and approached the activity of fibronectin(FN)-immobilized film. The RGDS-immobilized film was more stable against heat treatment and pH variation than the FN-immobilized film. In addition, the RGDS-immobilized film enhanced cell growth more strongly than the FN-immobilized film.Keywords
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