SEPARATION OF HUMAN EOSINOPHILS IN DENSITY GRADIENTS OF POLYVINYLPYRROLIDONE-COATED SILICA-GEL (PERCOLL)
- 1 January 1980
- journal article
- research article
- Vol. 40 (1) , 133-136
Abstract
A method for isolation of eosinophils from human peripheral blood using isosmolar solutions of polyvinylpyrrolidone-coated silica gel (Percoll) is described. The purity ranged from 86-99% eosinophils in the final preparation and the recovery was 38-56%. The separation technique did not affect the viability or the metabolic capacities of the cells.This publication has 9 references indexed in Scilit:
- Characterization of functionally distinct lymphoid and myeloid cells from human blood and bone marrow. I. Separation by a buoyant density gradient techniqueJournal of Immunological Methods, 1977
- OXIDATIVE-METABOLISM OF HUMAN EOSINOPHIL1977
- GRANULOCYTE FUNCTION IN CHRONIC GRANULOCYTIC LEUKEMIA .2. BACTERICIDAL CAPACITY, PHAGOCYTIC RATE, OXYGEN-CONSUMPTION, AND GRANULE PROTEIN-COMPOSITION IN ISOLATED GRANULOCYTES1976
- Production of Monospecific Rabbit Antihuman Eosinophil Serums and Demonstration of a Blocking PhenomenonNew England Journal of Medicine, 1974
- Selective stimulation and purification of eosinophils and neutrophils from guinea pig peritoneal fluids.1973
- SEPARATION OF HELA CELLS BY COLLOIDAL SILICA DENSITY GRADIENT CENTRIFUGATIONThe Journal of cell biology, 1972
- Myeloperoxidase‐Mediated Iodination in GranulocytesScandinavian Journal of Haematology, 1972
- Eosinophil cell separation from human peripheral blood.1970
- A method for obtaining concentrates of eosinophils from bloodJournal of Clinical Pathology, 1962