Studies of Nickel Carcinogenesis: Fractionations of Nickel in Ultracentrifugal Supernatants of Lung and Liver by Means of Dextran Gel Chromatography

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Abstract
Chromatographic fractiona-tions of ultracentrifugal supernatants of homogenates of rat lung and liver by use of columns of dextran gel (Sephadex G-100) are observed. A major proportion of nickel in these tissue supernatants is firmly bound to macromolecular constituents. Following acute and chronic inhalation of carcinogenic levels of nickel carbonyl, the predominant increases in the concentrations of nickel are observed in the macro-molecular fractions. These findings are consistent with the previous demonstration of nickel in purified preparations of RNA from several rat tissues, and with the observation of increased concentrations of nickel in high-molecular weight RNA from lung and liver following the inhalation of nickel carbonyl.