Genetic polymorphism of human plasma ?1-B-glycoprotein: phenotyping by immunoblotting or by a simple method of 2-D electrophoresis
- 1 June 1987
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 76 (2) , 111-115
- https://doi.org/10.1007/bf00284904
Abstract
Genetic polymorphism of human plasma (serum) α1B-glycoprotein (α1B) was observed using one-dimensional horizontal polyacrylamide gel electrophoresis (PAGE) pH 9.0 of plasma samples followed by Western blotting with specific antiserum to α1B. A simple method of two-dimensional agarose gel electrophoresis (pH 5.4) — horizontal PAGE (pH 9.0) of plasma samples, followed by general protein staining, was reported as an alternative method for α1B typing. The there different phenotypes of α1B observed (designated 1-1, 1-2, and 2-2) were apparently identical to those reported by Altland et al. (1983), who used double one-dimensional electrophoresis. Family data supported the hypothesis that the three α1B phenotypes are determined by two codominant alleles at an autosomal locus, designated A1B. Allele frequencies in a Swedish population were: A1B *1, 0.937; A1B *2, 0.063; PIC, 0.111. For clues on linkage relationships of human A/B, the previously known linkages of A1B in pigs and horses, including the one between A1B and the gene that determines susceptibility to malignant hyperthermia in pigs were discussed.This publication has 22 references indexed in Scilit:
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