Interactions among three distinct CesA proteins essential for cellulose synthesis
Top Cited Papers
- 21 January 2003
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 100 (3) , 1450-1455
- https://doi.org/10.1073/pnas.0337628100
Abstract
In a screen to identify novel cellulose deficient mutants, three lines were shown to be allelic and define a novel complementation group, irregular xylem5 ( irx5 ). IRX5 was cloned and encodes a member of the CesA family of cellulose synthase catalytic subunits (AtCesA4). irx5 plants have an identical phenotype to previously described mutations in two other members of this gene family ( IRX1 and IRX3 ). IRX5, IRX3, and IRX1 are coexpressed in exactly the same cells, and all three proteins interact in detergent solubilized extracts, suggesting that three members of this gene family are required for cellulose synthesis in secondary cell walls. The association of IRX1 and IRX3 was reduced to undetectable levels in the absence of IRX5. Consequently, these data suggest that IRX5, IRX3, and IRX1 are all essential components of the cellulose synthesizing complex and the presence of all three subunits is required for the correct assembly of this complex.Keywords
This publication has 24 references indexed in Scilit:
- Resistance against Herbicide Isoxaben and Cellulose Deficiency Caused by Distinct Mutations in Same Cellulose Synthase Isoform CESA6Plant Physiology, 2002
- PROCUSTE1 Encodes a Cellulose Synthase Required for Normal Cell Elongation Specifically in Roots and Dark-Grown Hypocotyls of ArabidopsisPlant Cell, 2000
- Multiple Cellulose Synthase Catalytic Subunits Are Required for Cellulose Synthesis in ArabidopsisPlant Cell, 2000
- Immunogold Labeling of Rosette Terminal Cellulose-Synthesizing Complexes in the Vascular Plant Vigna angularisPlant Cell, 1999
- The irregular xylem3 Locus of Arabidopsis Encodes a Cellulose Synthase Required for Secondary Cell Wall SynthesisPlant Cell, 1999
- β-Subunit Assembly Is Essential for the Correct Packing and the Stable Membrane Insertion of the H,K-ATPase α-SubunitPublished by Elsevier ,1999
- Molecular Analysis of Cellulose Biosynthesis in ArabidopsisScience, 1998
- The Plasma Membrane of Arabidopsis thaliana Contains a Mercury-Insensitive Aquaporin That Is a Homolog of the Tonoplast Water Channel Protein TIPPlant Physiology, 1994
- Transport of rosettes from the golgi apparatus to the plasma membrane in isolated mesophyll cells ofZinnia elegans during differentiation to tracheary elements in suspension cultureProtoplasma, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970