Effect of N-Alkyl and N-Alkenyl Substituents in Noroxymorphindole, 17-Substituted-6,7-dehydro-4,5α-epoxy-3,14-dihydroxy-6,7:2‘,3‘-indolomorphinans, on Opioid Receptor Affinity, Selectivity, and Efficacy

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Abstract
The N-alkyl analogues (N-ethyl through N-heptyl), branched N-alkyl chain analogues (N-isopropyl, N-2-methylpropyl, and N-3-methylbutyl), and N-alkenyl analogues ((E)-N-3-methylallyl (crotyl), N-2-methylallyl, and N-3,3-dimethylallyl) were prepared in the noroxymorphindole series (17-substituted-6,7-dehydro-4,5α-epoxy-3,14-dihydroxy-6,7:2‘,3‘-indolomorphinans), and the effect of the N-substituent on opioid receptor affinity, selectivity, and efficacy was examined using receptor binding assays, [35S]GTPγS efficacy determinations, and smooth muscle functional assays (electrically stimulated mouse vas deferens and guinea pig ileum). All of the compounds acted as opioid antagonists, including those with N-substituents which usually confer either weak agonist−antagonist behavior (N-ethyl) or potent opioid agonist activity (N-pentyl) in morphinan-like ligands which interact with the μ-receptor. Several N-substituted noroxymorphindoles were found to be more μ/δ-selective than naltrindole (NTI). The N-2-methylallylnoroxymorphindole, in particular, was found to be more selective than NTI in receptor binding assays (μ/δ = 1700 vs 120; κ/δ = 810 vs 140), as an antagonist in the GTPγS assay (μ/δ = 170 vs 140; κ/δ = 620 vs 160), and considerably more selective than NTI in the functional assays (μ/δ > 2200 vs 90). It also had high affinity for the δ-opioid receptor (Ki = 4.7 nM in the binding assay) and high antagonist potency (1.2 nM in the GTPγS assay; 8.9 nM in the MVD assay).

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