Stimulation by T Cell Independent Antigens Can Relieve the Arrest of Differentiation of Immature Auto‐Reactive B Cells in the Bone Marrow*
- 1 July 1995
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 42 (1) , 21-33
- https://doi.org/10.1111/j.1365-3083.1995.tb03621.x
Abstract
The pair of μH‐chain and kL‐chain transgenes encoding the Sp6 TNP/DN A‐specific IgM was bred onto the rearrangement‐deficient genetic background of RAG‐2T mice, and onto the kL‐chain expression‐deficient background of iEkT mice. Bone marrow of Sp6 transgenic RAG‐2T mice contained normal numbers of B220(CD45R)+ c‐kit+ pro/preB‐I‐like cells and normal numbers of B220(CD45R)+TAC+ preB‐II‐like cells. Most strikingly, the numbers of immature sIgM+ B cells in the bone marrow were at least five‐fold lower than normal, while mature B cells were almost undetectable in bone marrow as well as spleen. Hence, B cell development in these mice appears to be arrested at the transition from preB‐II to immature B cells. The contents of bone marrow and spleen of the different precursors, immature and mature B cell compartments in Sp6iEkT mice were found to be similar to those of normal mice except that all sIg+ cells expressed μL‐chains, of which 40% coexpressed the transgenic kL‐chain. It indicates that the repertoire of μL‐chain rearrangements and the μL‐chains expressed from it suffices to relieve the arrest of differentiation seen in Sp6RAG‐2T mice. The T cell‐independent antigen TNP‐Ficoll elicited within 5 days a response of the Sp6RAG‐2T mice to develop to IgM‐secreting cells and to fill the serum pool with the Sp6 transgenic IgM to 100 μg/ml, i. e. to normal serum levels of IgM in normal mice. TNP‐Ficoll appears to interfere with the arrest of differentiation. Two scenarios for this arrest of differentiation and its relief by the T‐independent antigen TNP‐Ficoll are discussed.Keywords
This publication has 44 references indexed in Scilit:
- Two Pathways of B‐Lymphocyte Development in Mouse Bone Marrow and the Roles of Surrogate L Chain in this DevelopmentImmunological Reviews, 1994
- Positive selection of lymphocytesCell, 1994
- Suppression of B cell differentiation by ligation of membrane‐bound IgMEuropean Journal of Immunology, 1993
- A transgenic model of autoimmune hemolytic anemia.The Journal of Experimental Medicine, 1992
- Evidence for regulation of naturally activated autoreactive B cellsEuropean Journal of Immunology, 1991
- Selective peripheral expansion and activation of B cells expressing endogenous immunoglobulin in μ-transgenic miceEuropean Journal of Immunology, 1990
- Immune status of a μ, χ transgenic mouse line. Deficient response to bacterially related antigensEuropean Journal of Immunology, 1989
- Expression of immunoglobulin delta chain causes allelic exclusion in transgenic miceNature, 1987
- Transmission and expression of a specific pair of rearranged immunoglobulin μ and κ genes in a transgenic mouse lineNature, 1985
- Immunoglobulin M mutantsEuropean Journal of Immunology, 1980