Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family
- 26 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 570 (1-3) , 217-222
- https://doi.org/10.1016/j.febslet.2004.06.038
Abstract
The citM gene from Lactococcus lactis CRL264 was demonstrated to encode for an oxaloacetate decarboxylase. The enzyme exhibits high levels of similarity to malic enzymes (MEs) from other organisms. CitM was expressed in Escherichia coli , purified and its oxaloacetate decarboxylase activity was demonstrated by biochemical and genetic studies. The highest oxaloacetate decarboxylation activity was found at low pH in the presence of manganese, and the K m value for oxaloacetate was 0.52 ± 0.03 mM. However, no malic activity was found for this enzyme. Our studies clearly show a new group of oxaloacetate decarboxylases associated with the citrate fermentation pathway in gram-positive bacteria. Furthermore, the essential catalytic residues were found to be conserved in all members of the ME family, suggesting a common mechanism for oxaloacetate decarboxylation. Keywords Citrate fermentation Lactic acid bacteria Malic enzyme Oxaloactetate decarboxylase Abbreviations ME, malic enzyme IPTG, isopropyl-β- d -thiogalactopyranoside OAA, oxaloacetic acid LDH, l -lactate dehydrogenaseKeywords
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