High efficiency electroporation of Lactococcus lactis subsp. lactis LM0230 with plasmid pGB301
- 1 August 1990
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 11 (2) , 62-64
- https://doi.org/10.1111/j.1472-765x.1990.tb01275.x
Abstract
Electroporation-mediated transformation of Lactococcus lactis with plasmid pGB301, a 9.8 kilobase pair vector (Behnke et al. 1981), has been reported by McIntyre & Harlander (1989a). Improved transformation efficiencies of 10(2)-10(3)/micrograms DNA were achieved by altering the conditions under which the bacteria were grown prior to electroporation (McIntyre & Harlander 1989b). This present investigation sought to improve still further transformation efficiencies in order to provide a reliable high frequency transformation system for Lc. lactis subsp. lactis.Keywords
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