Similarity of minus origins of replication and flanking open reading frames of plasmids pUB110, pTB913 and pMV158
Open Access
- 1 January 1989
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (18) , 7283-7294
- https://doi.org/10.1093/nar/17.18.7283
Abstract
Plasmids pMV 158 and pTB913, originating from Streptococcus agalactiae and a thermophilic Bacillus respectively, were sequenced to completion. Both contained a BA3-type minus origin of replication and an RSA-site, believed to constitute a site-specific recombination site. These two regions were more than 99% homologous to the corresponding regions of the Staphylococcus aureus plasmid pUB110. Deleting the BA3-type minus origin resulted in the accumulation of a considerable amount of single-stranded DNA, both in L.lactis subsp. lactis and B.subtilis, indicating that this minus origin was functional in both bacterial species. Like pUB110, both plasmids contained an open reading frame encoding a putative plasmid recombinalion enzyme (Pre protein), which was located downstream of the RSA-site. On the basis of sequence comparisons between pUB110, pMV158, pTB913, pT181, pE194, pNE131 and pT48 two distinct families of RSA-sites and Pre proteins could be distinguishedKeywords
This publication has 35 references indexed in Scilit:
- The Nucleotide Sequence of Staphylococcus aureus Plasmid pT48 Conferring Inducible Macrolide-Lincosamide-Streptogramin B Resistance and Comparison with Similar Plasmids Expressing Constitutive ResistanceMicrobiology, 1988
- Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1Journal of Molecular Biology, 1986
- The nucleotide sequence of pUB110: Some salient features in relation to replication and its regulationPlasmid, 1986
- Alkaline transfer of DNA to plastic membraneBiochemical and Biophysical Research Communications, 1984
- PROTEIN-DNA RECOGNITIONAnnual Review of Biochemistry, 1984
- Two Replication Determinants of an Antibiotic-resistance Plasmid, pTB19, from a Thermophilic BacillusMicrobiology, 1984
- Two plasmid-determined restriction and modification systems in Streptococcus lactisPlasmid, 1984
- Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fdGene, 1980
- Ultraviolet inactivation and excision-repair in Bacillus subtilis I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivativesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970