Loss of function mutation in the yeast multiple drug resistance gene PDR5 causes a reduction in chloramphenicol efflux
- 1 October 1994
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 38 (10) , 2492-2494
- https://doi.org/10.1128/aac.38.10.2492
Abstract
The yeast (Saccharomyces cerevisiae) PDR5 gene product encodes a 160-kDa protein related to the large ABC family of transporters, including the human MDR1 multidrug resistance p-glycoprotein. Loss of function mutations in PDR5 result in chloramphenicol hypersensitivity. A pdr5::Tn5 loss of function mutant exhibits a markedly impaired efflux of chloramphenicol compared with that of an isogenic PDR5 (wild-type) control.Keywords
This publication has 10 references indexed in Scilit:
- Active efflux of chloramphenicol in susceptible Escherichia coli strains and in multiple-antibiotic-resistant (Mar) mutantsAntimicrobial Agents and Chemotherapy, 1994
- PDR5, a novel yeast multidrug resistance conferring transporter controlled by the transcription regulator PDR1.Journal of Biological Chemistry, 1994
- Separation of drug transport and chloride channel functions of the human multidrug resistance P-glycoproteinCell, 1992
- Interaction of the yeast pleiotropic drug resistance genes PDR1 and PDR5Current Genetics, 1992
- Several alleles of the multidrug-resistance gene are closely linked to chloroquine resistance in Plasmodium falciparumNature, 1990
- Chloroquine resistance not linked to mdr-like genes in a Plasmodium falciparum crossNature, 1990
- Amplification of a Gene Related to Mammalian mdr Genes in Drug-Resistant Plasmodium falciparumScience, 1989
- Reversal of Chloroquine Resistance in Plasmodium falciparum by VerapamilScience, 1987
- The detection and classification of membrane-spanning proteinsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1985
- REDUCED DRUG ACCUMULATION IN MULTIPLY DRUG-RESISTANT HUMAN-KB CARCINOMA CELL-LINES1985