Amber dnaG mutation exerting a polar effect on the synthesis of RNA polymerase sigma factor in Escherichia coli
- 1 August 1984
- journal article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 196 (1) , 179-182
- https://doi.org/10.1007/bf00334114
Abstract
Three amber mutants of Escherichia coli, dnaG9, dnaG24 and dnaG26, affected in the structural gene (dnaG) for “primase” have been isolated from a parental strain carrying a temperature-sensitive amber suppressor (supF-Ts6). These mutants grow at 30° C but not at 42° C since primase is essential for growth and is synthesized only at low temperatures. Chimeric plasmids carrying dnaG + but no other chromosomal genes of E. coli complemented the amber mutations, and the plasmid carrying a part of dnaG lost the complementing activity. Beside, plasmids carrying a dnaG amber mutation complemented a temperature-sensitive dnaG mutation only in the presence of amber suppressor. One of the amber mutation, dnaG24 which maps proximal to the NH2-terminus of the dnaG gene, exerted a polar effect on the synthesis of RNA polymerase σ factor in E. coli.Keywords
This publication has 19 references indexed in Scilit:
- Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12Molecular Genetics and Genomics, 1983
- The operen that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12Cell, 1983
- Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.Proceedings of the National Academy of Sciences, 1982
- The nudeotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E. coil K12Nucleic Acids Research, 1981
- Amber mutations in the structural gene for RNA polymerase sigma factor of Escherichia coliMolecular Genetics and Genomics, 1980
- Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.Proceedings of the National Academy of Sciences, 1979
- Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase.Proceedings of the National Academy of Sciences, 1979
- A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.Proceedings of the National Academy of Sciences, 1977
- Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.Proceedings of the National Academy of Sciences, 1977
- RNA POLYMERASEAnnual Review of Biochemistry, 1971