Southern molecular hybridization experiments with parallel complementary DNA probes
- 10 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 297 (3) , 233-236
- https://doi.org/10.1016/0014-5793(92)80545-r
Abstract
We have detected the specific binding in Southern blot hybridization experiments of both complementary antiparallel and parallel 40 bp synthetic DNA probes, corresponding to a cloned Drosophila DNA fragment. The highly cooperative annealing and melting were observed in solution with these probes, which are complementary in the same direction and possess 17 GC pairs. The binding of ethidium bromide is indicative of formation of a perfect parallel DNA duplex. The specific binding was also detected in both genomic and in plaque hybridization experiments.Keywords
This publication has 13 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- A fluorescent complex between ethidium bromide and nucleic acids: Physical—Chemical characterizationPublished by Elsevier ,2004
- Mobile elements and transposition events in the cut locus of Drosophila melanogasterMolecular Genetics and Genomics, 1989
- Parallel stranded duplex DNANucleic Acids Research, 1988
- Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.Proceedings of the National Academy of Sciences, 1985
- Hybridization of nucleic acids directly in agarose gelsAnalytical Biochemistry, 1983
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965
- SEQUENCE COMPLEMENTARITY OF T2-DNA AND T2-SPECIFIC RNAProceedings of the National Academy of Sciences, 1961
- EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTSProceedings of the National Academy of Sciences, 1957