Cytological effects on Chinese hamster cells of synchronizing concentrations of hydroxyurea

Publisher Website
Google Scholar
Abstract
The cytological effects of 2 mM hydroxyurea upon Chinese hamster cells at various phases of the cell cycle were examined. Cells in the G1, G2, or M phases of the generation cycle treated with hydroxyurea showed no chromosomal aberrations. Cell treated in S phase became moribund and eventually lysed. Some of these moribund S cells reached mitosis much later and were found to have chromatid aberrations. Cells in the log phase of growth, surviving exposure to 2 mM hydroxyurea for six hours, also showed no aberrations. Thus, viable (colony‐forming) cells, resulting from synchrony procedures with hydroxyurea are free of chromosomal aberrations.