BARLEY CULTIVAR IDENTIFICATION BY SDS GRADIENT PAGE ANALYSIS OF HORDEIN

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Abstract
Sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis (SDSGPAGE) was evaluated to determine whether it could provide improved resolving power of hordein proteins concomitant with improved discrimination (identification) of Canadian barley cultivars. Hordeins were subjected to electrophoresis using a linear gradient gel and a discontinuous buffer system. Proteins were detected using a silver-based color stain and gels were calibrated routinely for molecular weight determinations with a mixture of standard proteins. Molecular weight estimations of hordeins were performed by evaluation of the relationship between log (MW) versus log (R1) and reproducible linear calibration curves and molecular weight estimates for hordeins (CV 0.66%) were obtained. Densitometric analysis of protein band intensities revealed considerable variability in protein staining. Electrophoretic analysis of 100 barley cultivars revealed that, in general, resolution of SDSGPAGE was superior to that of acidic PAGE but considerable duplication of protein patterns among cultivars and their biotypes remained a problem. Cultivar formulae consisting of B, C and D hordein pattern designations were used to prepare a cultivar catalogue of protein patterns. Sixty-five formulae consisting of 30 B, 36 C and 3 D hordein patterns were obtained. SDSGPAGE also was found suitable for barley malt cultivar analysis.Key words: SDS gradient PAGE, barley, cultivar identification, hordein

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