The Rex regulatory protein of human T-cell lymphotropic virus type I binds specifically to its target site within the viral RNA.
- 15 August 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (16) , 7145-7149
- https://doi.org/10.1073/pnas.88.16.7145
Abstract
The Rex protein of human T-cell leukemia virus type I (HTLV-I) was expressed in bacteria and partially purified. Rex was shown to bind in vitro specifically to an RNA sequence located in the 3' long terminal repeat of HTLV-I, named Rex-responsive element (RXRE). Rex also bound in vitro to the human immunodeficiency virus type 1 (HIV-1) Rev-responsive element (RRE), while purified HIV-1 Rev protein did not bind to the RXRE. The binding results obtained in vitro are therefore in agreement with the nonreciprocal function of Rev and Rex in vivo. Rex binds specifically to both RRE and RXRE and activates expression in both HIV-1 and HTLV-I, while Rev binds to RRE and activates only HIV-1. Binding of Rex to RRE deletion mutants previously shown to lack either the Rev-responsive or the Rex-responsive portion suggested preferential binding of Rex to a distinct target within the RRE. These results demonstrated that Rex, like Rev, acts by binding to a specific RNA target.Keywords
This publication has 36 references indexed in Scilit:
- Structure-function analyses of the HTLV-I Rex and HIV-1 Rev RNA response elements: insights into the mechanism of Rex and Rev action.Genes & Development, 1990
- Regulation of HIV-1 Gene ExpressionAnnual Review of Immunology, 1990
- Secondary Structure is the Major Determinant for Interaction of HIV rev Protein with RNAScience, 1990
- Functional Analysis of CAR, the Target Sequence for the Rev Protein of HIV-1Science, 1989
- Sequence-specific RNA binding by the HIV-1 Rev proteinNature, 1989
- Comparative analysis of the HTLV-I Rex and HIV-1 Rev trans-regulatory proteins and their RNA response elements.Genes & Development, 1989
- Trans-dominant inactivation of HTLV-I and HIV-1 gene expression by mutation of the HTLV-I Rex transactivatorNature, 1989
- The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNANature, 1989
- The second pX product p27x‐III of HTLV‐1 is required for gag gene expressionFEBS Letters, 1986
- Electrophoretic separation of complexes involved in the splicing of precursors to mRNAsPublished by Elsevier ,1986