L-Serine Production by Temperature-sensitive Mutants of Methanol-utilizing BacteriumPseudomonasMS 31
- 1 June 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 45 (6) , 1425-1430
- https://doi.org/10.1080/00021369.1981.10864730
Abstract
Temperature-sensitive mutants producing L-serine efficiently from glycine were obtained from the facultative methylotroph Pseudomonas MS 31. Forty-five mutant strains showed adequate growth on methanol at 30°C but little or no growth at 37°C. Fourteen of these mutants produced L- serine more efficiently than the wild-type strain. The typical mutant strain ts 162 showed a high conversion rate in glycine-to-L-serine when the cultivation temperature was changed from a permissive (30°C) to non-permissive state (38˜42°C) together with the addition of glycine and methanol after adequate growth. The mutant strain accumulated 6.8 mg L-serine from 12 mg glycine per ml culture under optimum conditions. The reduction of L-serine degrading activity in the mutant strain seemed to contribute to the high productivity of L-serine.This publication has 1 reference indexed in Scilit:
- L-Serine Production by Methanol-utilizing BacteriumPseudomonasMS 31Agricultural and Biological Chemistry, 1981