The role of DNA double strand breaks in lonizing radiation‐induced killing of eukaryotic cells
- 1 December 1991
- Vol. 13 (12) , 641-648
- https://doi.org/10.1002/bies.950131204
Abstract
A widely accepted assumption in radiobiology is that ionizing radiation kills cells by inducing forms of damage in DNA structures that lead to the formation of lethal chromosome aberrations. One goal of radiation biology research is the identification of these forms of DNA damage, the characterization of the mechanisms involved in their repair and the elucidation of the processes involved in their transformation to chromosome damage, In recent years, evidence has accumulated implicating DNA double stranded breaks as lesions relevant for cell killing. Here, the available information on this topic is reviewed together with the methods most commonly used to quantitate induction and repair of this type of lesion. The presentation concludes with an outline of present research directions and future goals.Keywords
This publication has 68 references indexed in Scilit:
- Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cellsRadiation and Environmental Biophysics, 1990
- Saturable Repair Models of Radiation Action in Mammalian CellsRadiation Research, 1985
- The molecular nature of mutations in cultured mammalian cells: A reviewMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984