Spore Production and Artificial Inoculation Techniques forGremmeniella abietina

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Abstract
Viable conidia of Brunchorstia-pinea (teleomorph = Gremmeniella-abietina) were produced in flasks containing 20% V-8 broth incubated at 16-18 C under a 16-hr photoperiod with a light intensity of approximately 100 .mu.E m-2 sec-1. More than 6 .times. 107 spores per flask were usually produced in 21 days under these conditions. Red pine seedlings were inoculated with a suspension of conidia that had been produced in this way, held at 18 C at high relative humidity for 3 days, and transferred to 4 C. Disease symptoms of Scleroderris shoot blight, as indicated by loose fascicles, appeared after as few as 56 days in the dark at 4C.

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