Selective loss of uncoupling protein mRNA in brown adipose tissue on deacclimation of cold-acclimated mice
- 1 November 1987
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 65 (11) , 955-959
- https://doi.org/10.1139/o87-124
Abstract
The time course of changes in the level of uncoupling protein mRNA when cold-acclimated mice were returned to a thermoneutral environment (33 °C) was examined using a cDNA probe. Upon deacclimation, there was a marked loss of uncoupling protein mRNA within 24 h, which precedes the loss of uncoupling protein from mitochondria. This loss of uncoupling protein mRNA was selective, since there was no change in the relative proportion of cytochrome c oxidase subunit IV mRNA or poly(A)+ RNA in total RNA. The results suggest that the decrease in the mitochondrial content of uncoupling protein during deacclimation is likely the result of turnover of existing protein, with very little replacement due to a lower level of its mRNA.This publication has 17 references indexed in Scilit:
- Fatty acid activation of the reconstituted brown adipose tissue mitochondria uncoupling protein.Journal of Biological Chemistry, 1985
- Increase of uncoupling protein and its mRNA in brown adipose tissue of rats fed on ‘cafeteria diet’Biochemical Journal, 1985
- Mitochondrial thermogenin content is unchanged during atrophy of BAT of fasting miceAmerican Journal of Physiology-Endocrinology and Metabolism, 1985
- Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.Proceedings of the National Academy of Sciences, 1985
- Increased level of mRNA for the uncoupling protein in brown adipose tissue of rats during thermogenesis induced by cold exposure or norepinephrine infusion.Journal of Biological Chemistry, 1984
- Parallel increases in amount of (3H)GDP binding and thermogenin antigen in brown-adipose-tissue mitochondria of cafeteria-fed ratsBiochemical and Biophysical Research Communications, 1984
- Brown‐adipose‐tissue mitochondria: the regulation of the 32 000‐Mr uncoupling protein by fatty acids and purine nucleotidesEuropean Journal of Biochemistry, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- A role for brown adipose tissue in diet-induced thermogenesisNature, 1979