Effect of Methotrexate on Folate Binding to a Folate Binding Protein in Cow's Milk
- 1 January 1978
- journal article
- research article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 42 (1) , 77-80
- https://doi.org/10.1111/j.1600-0773.1978.tb02172.x
Abstract
Folate in cow's milk was strongly bound to a minor whey protein. Methotrexate inhibited the folate binding in a log dosis dependent manner, but was a rather weak inhibitor. The binding mechanism of folate changed from a cooperative to a non-cooperative type in the presence of methotrexate. This model system could be used in investigations on human body fluids and tissues.Keywords
This publication has 5 references indexed in Scilit:
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- On the fractionation of β-lactoglobulin and α-lactalbuminBiochimica et Biophysica Acta (BBA) - Protein Structure, 1967