Detection of a novel HLA-DQ specificity: serological and immunochemical analyses by a monoclonal antibody
- 1 September 1987
- journal article
- research article
- Published by Springer Nature in Immunogenetics
- Vol. 26 (3) , 143-149
- https://doi.org/10.1007/bf00365903
Abstract
A monoclonal antibody (mAb) with a novel human B-cell allospecificity was produced by immunizing a C3H/He mouse with the human B lymphoblastoid cell line EBV-Wa (HLA-DR4/Dw15/DQblank homozygous). The mAb, termed HU-46, reacted with B cells from not only DR4/Dw15-positive individuals but also certain DRw8/Dw8-positive ones whose DQ phenotypes had not yet been defined. Two-dimensional gel analyses indicated that the mAb recognized class II antigens which were encoded by the HLA-DQ locus. Furthermore, in genetic analysis, the gene encoding the class II antigen detected by HU-46 met the Hardy-Weinberg condition as a fourth allele of the DQ locus. We provisionally labeled this novel DQ specificity DQWa.This publication has 28 references indexed in Scilit:
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