Antigen-presenting cell function in induction of helper T cells for cytotoxic T-lymphocyte responses: evidence for antigen processing.

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Abstract
Splenic adherent lls (SAC) apparently play an active role in the presentation of H-2Kk antigen for an alloreactive [mouse] cytotoxic T lymphocyte (CTL) response. If antigen is incubated with SAC for 12 h, they will provide maximal stimulation and present the antigen in the contex of their Ia molecules. UV irradiation of these SAC, prior to the 12 h incubation with H-2Kk antigen, abrogates this stimulatory capacity. Macrophage-bound antigen is not sufficient for stimulation of a response; a 2nd signal is required as well, that in this system, is provided by phorbol myristate acetate. The SAC are involved in the activation of helper T cells; they are not required for presentation of antigen to the precytotoxic T lymphocyte, which requires 2 signals for activation, provided by antigen and the other by a T cell-derived helper factor.