MACROPHAGE-MIGRATION INHIBITION STUDIES OF LYMPHOCYTES TAKEN FROM GUINEA-PIGS SUFFERING FROM EXPERIMENTAL POLYMYOSITIS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 25  (1) , 133-138
Abstract
An experimental form of polymyositis, induced in guinea-pigs by injections of rabbit muscle homogenate in Freund''s complete adjuvant (FCA), was investigated to analyze the role of cellular immune response in this disease. The direct macrophage migration inhibition test on peritoneal exudate cells taken from myositic animals, was used to identify those components of mucle against which lymphocytes of myositic animals are sensitized. These lymphocytes were sensitized principally against the myofibrillar fraction of muscle. When tested against purified myofibrillar proteins, they were strongly sensitized against myosin and tropomyosin, but not against troponin. A similar migration inhibition technique is being developed for use as a diagnostic and investigatory tool in the study of human polymyositis.