Construction and characterization of an Escherichia coli plasmid bearing a functional gene G of bacteriophage phiX174.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2) , 774-778
- https://doi.org/10.1073/pnas.75.2.774
Abstract
To study the mutagenic effects of site-specific, covalent modifications of biologically active DNA, host cells that are permissive for any type of mutation that might be produced in vivo from the modified DNA are needed. Specifically, a general in vivo complementation system for the bacteriophage .vphi.X174 gene G, an essential gene that was chosen for initial studies of chemical mutagenesis, is required. A plasmid (p.vphi.XG) that carries a functional copy of .vphi.X174 gene G was constructed. Three different bacterial strains that are nonpermissive for am9, a gene G amber mutant, were transformed with p.vphi.XG. The transformants are now permissive for this gene G mutant, but not for the gene A or E mutants that were tested. This paper describes the construction and the biochemical characterization of this plasmid, p.vphi.XG, and describes some of the biological properties exhibited by the p.vphi.XG-bearing strains.This publication has 25 references indexed in Scilit:
- Completed DNA sequences and organization of repressor-binding sites in the operators of phage lambdaJournal of Molecular Biology, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.1976
- Transcription of bacteriophage φX174 in vitro: Analysis with restriction enzymesJournal of Molecular Biology, 1976
- A general method for cloning eukaryotic structural gene sequences.Proceedings of the National Academy of Sciences, 1976
- Cleavage map of bacteriophage X174 RF DNA by restriction enzymesNucleic Acids Research, 1976
- The in vitro transcription units of bacteriophage φX174Journal of Molecular Biology, 1976
- Amplification and characterization of a β-globin gene synthesized in vitroCell, 1976
- Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynudeotidyl transferaseNucleic Acids Research, 1976
- Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals.Proceedings of the National Academy of Sciences, 1975