A Study of the Growth of Excised Embryo Shoot Apices of Wheat In vitro

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Abstract
A technique for obtaining excised embryo shoot apices of wheat is described. When yeast extract or various amino acids were included in the basal medium the explants did not grow and much of the tissue showed signs of necrosis. Coconut milk, either autoclaved or filter-sterilized, inhibited the growth of the explants. Root initiation and shoot growth were stimulated by ammonium salts. It was not known whether root initiation was the result of stimulation by ammonium salts or the rapid growth of the shoot apex. Aqueous extracts of roots also stimulated the growth of the explants, but extracts of other plant organs did not. The addition of individual vitamins had various effects on the growth of the explants, but since the growth response was so slow, results other than complete inhibition were rejected. On the basis of these results, other substances appear to be necessary to support the growth of wheat explants without the initiation of roots.

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