Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.
Open Access
- 1 March 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (3) , 1103-1112
- https://doi.org/10.1128/mcb.8.3.1103
Abstract
Poliovirus polysomal RNA is naturally uncapped, and as such, its translation must bypass any 59 cap-dependent ribosome recognition event. To elucidate the manner by which poliovirus mRNA is translated, we have determined the translational efficiencies of a series of deletion mutants within the 59 noncoding region of the mRNA. We found striking differences in translatability among the altered mRNAs when assayed in mock-infected and poliovirus-infected HeLa cell extracts. The results identify a functional cis-acting element within the 59 noncoding region of the poliovirus mRNA which enables it to translate in a cap-independent fashion. The major determinant of this element maps between nucleotides 320 and 631 of the 59 end of the poliovirus mRNA. We also show that this region (320 to 631), when fused to a heterologous mRNA, can function in cis to render the mRNA cap independent in translation. ImagesThis publication has 40 references indexed in Scilit:
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