2-Deoxy-d-glucose Inhibits Rejoining of Radiation-induced DNA Double-strand Breaks in Yeast
- 1 January 1992
- journal article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 61 (2) , 185-190
- https://doi.org/10.1080/09553009214550801
Abstract
Effects of 2-deoxy-D-glucose (2-DG) on radiation-induced DNA double-strand breaks (dsb) have been studied under non-growth conditions in a respiratory-deficient strain of the yeast Saccharomyces cerevisiae. Velocity sedimentation in neutral sucrose gradients was used to measure DNA dsb. Addition of 2-DG to the liquid-holding medium (67 mM phosphate buffer, pH 5, 30 degrees C) at an equimolar concentration with glucose (50 mM) reduced the rate and extent of dsb rejoining. The inhibition of rejoining mediated by 2-DG is reversible for the majority--but not all--of the radiation-induced dsb.Keywords
This publication has 23 references indexed in Scilit:
- A Comparative Study of Rejoining of DNA Double-strand Breaks in Yeast Irradiated with 3·5 MeV α-particles or with 30 MeV ElectronsInternational Journal of Radiation Biology, 1990
- Energy linked modifications of the radiation response in a human cerebral glioma cell lineInternational Journal of Radiation Oncology*Biology*Physics, 1989
- Alterations in Phosphate Metabolism during Cellular Recovery of Radiation Damage in YeastInternational Journal of Radiation Biology, 1988
- Potentially Lethal Damage, Sublethal Damage and DNA Double Strand BreaksRadiation Protection Dosimetry, 1985
- DNA strand breaks, repair, and survival in x-irradiated mammalian cells.Proceedings of the National Academy of Sciences, 1976
- Induction of DNA double-strand breaks by X-rays in a radiosensitive strain of the yeast Saccharomyces cerevisiaeMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
- Isolation and Characterization of Mutants of Saccharomyces cerevisiae Auxotrophic and Conditionally Auxotrophic for 5′ -dTMPZeitschrift für Naturforschung C, 1974
- Mechanisms of chromosomal aberration production III. Chemicals and ionizing radiationMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- A ferrous sulphate dosemeter independent of photon energy in the range from 25 keV up to 50 MeVPhysics in Medicine & Biology, 1969
- Metabolic studies with 2-deoxyhexoses☆I. Mechanisms of inhibition of growth and fermentation in baker's yeastBiochimica et Biophysica Acta (BBA) - General Subjects, 1964